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1995-03-04
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Document 0160
DOCN M9550160
TI Uses of flow cytometry in virology.
DT 9505
AU McSharry JJ; Department of Microbiology, Immunology and Molecular
Genetics,; Albany Medical College, New York 12208.
SO Clin Microbiol Rev. 1994 Oct;7(4):576-604. Unique Identifier : AIDSLINE
MED/95136209
AB This article reviews some of the published applications of flow
cytometry for in vitro and in vivo detection and enumeration of
virus-infected cells. Sample preparation, fixation, and permeabilization
techniques for a number of virus-cell systems are evaluated. The use of
flow cytometry for multiparameter analysis of virus-cell interactions
for simian virus 40, herpes simplex viruses, human cytomegalovirus, and
human immunodeficiency virus and its use for determining the effect of
antiviral compounds on these virus-infected cells are reviewed. This is
followed by a brief description of the use of flow cytometry for the
analysis of several virus-infected cell systems, including blue tongue
virus, hepatitis C virus, avian reticuloendotheliosis virus, African
swine fever virus, woodchuck hepatitis virus, bovine viral diarrhea
virus, feline leukemia virus, Epstein-Barr virus, Autographa californica
nuclear polyhedrosis virus, and Friend murine leukemia virus. Finally,
the use of flow cytometry for the rapid diagnosis of human
cytomegalovirus and human immunodeficiency virus in peripheral blood
cells of acutely infected patients and the use of this technology to
monitor patients on antiviral therapy are reviewed. Future prospects for
the rapid diagnosis of in vivo viral and bacterial infections by flow
cytometry are discussed.
DE Antibodies, Viral/ANALYSIS/ISOLATION & PURIF Antigens, Viral/ISOLATION
& PURIF *Flow Cytometry Forecasting Human Specimen Handling Stains
and Staining Support, U.S. Gov't, P.H.S. Virology/METHODS
Viruses/IMMUNOLOGY/*ISOLATION & PURIF/*PHYSIOLOGY JOURNAL ARTICLE
REVIEW REVIEW, TUTORIAL
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).